1GYK
Serum Amyloid P Component co-crystallised with MOBDG at neutral pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.760, 70.530, 103.410 |
Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sac |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.056 | 0.122 * |
Number of reflections | 66447 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 95.2 | 92.1 * |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13.5 (mg/ml) | |
2 | 1 | drop | MO beta DG | 37 (mM) | |
3 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
4 | 1 | drop | 140 (mM) | ||
5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
6 | 1 | reservoir | 140 (mM) | ||
7 | 1 | reservoir | calcium acetate | 10 (mM) | |
8 | 1 | reservoir | PEG550 MME | 10 (%(w/v)) |