1GYD
Structure of Cellvibrio cellulosa alpha-L-arabinanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 90.078, 90.078, 177.217 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 * - 2.050 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TRICLINIC STRUCTURE PREVIOUSLY SOLVED |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.13) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.056 | 0.565 |
Number of reflections | 27420 | |
<I/σ(I)> | 29.4 | 3.27 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.7 * | 6.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8 | 10% PEG8000 100MM TRIS-HCL PH8.0, 20% GLYCEROL, pH 8.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG8000 | 10 (%(w/v)) | |
2 | 1 | 1 | Tris-HCl | 100 (mM) | pH8.0 |
3 | 1 | 1 | glycerol | 20 (%(v/v)) | |
4 | 1 | 1 | protein | 20 (mg/ml) |