1GYD

Structure of Cellvibrio cellulosa alpha-L-arabinanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	90.078, 90.078, 177.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.050
R-factor	0.207
Rwork	0.205
R-free	0.25000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRICLINIC STRUCTURE PREVIOUSLY SOLVED
RMSD bond length	0.013
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.056	0.565
Number of reflections	27420
<I/σ(I)>	29.4	3.27
Completeness [%]	99.9	99.9
Redundancy	6.7 *	6.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8		10% PEG8000 100MM TRIS-HCL PH8.0, 20% GLYCEROL, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	10 (%(w/v))
2	1	1	Tris-HCl	100 (mM)	pH8.0
3	1	1	glycerol	20 (%(v/v))
4	1	1	protein	20 (mg/ml)