1GY5

D92N,D94N double point mutant of human Nuclear Transport Factor 2 (NTF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	35.040, 79.020, 42.140
Unit cell angles	90.00, 104.36, 90.00

Refinement procedure

Resolution	18.000 - 2.300
R-factor	0.222 *
Rwork	0.222
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.100
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.450	0.092 *
Total number of observations	18416 *
Number of reflections	7519
Completeness [%]	96.6	91.7 *
Redundancy	2.3	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	18 *	pH 4.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	8 (%)
2	1	reservoir	sodium acetate	100 (mM)	pH4.5
3	1	reservoir		50 (mM)
4	1	drop	protein	2.8 (mg/ml)