1GY0
crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM C (P3121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.466, 81.466, 77.536 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.000 - 2.080 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 17787 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 100 MM TRIS PH8.3, 200 MM LI2SO4, 22 % PEG4000, pH 8.30 |
