1GXZ

crystal structure of the eukaryotic mono-ADP-ribosyltransferase ART2.2; Crystal form B (P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	69.560, 77.280, 86.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 2.100
R-factor	0.181
Rwork	0.181
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.130
High resolution limit [Å]	2.000 *	2.020
Rmerge	0.063 *	0.180 *
Total number of observations	126778 *
Number of reflections	30925	4218 *
<I/σ(I)>	8.1	3.8
Completeness [%]	99.1	99.1
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3	293 *	Mueller-Dieckmann, C., (2002) Acta Crystallogr., D58, 1211. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.8 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)	pH8.3
3	1	reservoir		200 (mM)
4	1	reservoir	PEG4000	22 (%(w/v))