1GXY

crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM A (P21)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	46.510, 85.640, 57.960
Unit cell angles	90.00, 94.60, 90.00

Refinement procedure

Resolution	35.000 * - 1.700 *
R-factor	0.187
Rwork	0.187
R-free	0.22300 *
Structure solution method	SIRAS
RMSD bond length	0.007 *
RMSD bond angle	1.380 *
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	1.800
High resolution limit [Å]	1.700 *	1.710
Rmerge	0.034 *	0.119 *
Total number of observations	195638 *
Number of reflections	48705	6787 *
<I/σ(I)>	10.5	6
Completeness [%]	99.1	99.1
Redundancy	4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3	293 *	Mueller-Dieckmann, C., (2002) Acta Crystallogr., D58, 1211. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.8 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)	pH8.3
3	1	reservoir		200 (mM)
4	1	reservoir	PEG4000	22 (%(w/v))