1GXJ

SMC hinge domain from T. maritima w/o coiled coil

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Wavelength(s)	0.9793, 0.9798, 0.93
Spacegroup name	P 1 21 1
Unit cell lengths	54.700, 57.900, 62.500
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.226 *
Rwork	0.226
R-free	0.26700 *
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.135
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.072	0.185
Number of reflections	16848
<I/σ(I)>	12.2	4.3
Completeness [%]	95.0	93.7
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	9.2	19 *	pH 9.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	26 (%)
2	1	reservoir	CHES	0.1 (M)	pH9.2
3	1	drop	protein	20 (mg/ml)