1GWK

Carbohydrate binding module family29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Spacegroup name	P 43 21 2
Unit cell lengths	93.270, 93.270, 83.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.340
R-factor	0.196
Rwork	0.193
R-free	0.24000 *
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.11)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.086	0.267
Number of reflections	29468
<I/σ(I)>	18.45	6.75
Completeness [%]	99.8	100
Redundancy	5.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		100MM NA HEPES, 150MM KSCN 20% ETHYLENE GLYCOL, 20% PEG3350, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium HEPES	100 (mM)	pH7.5
3	1	reservoir		150 (mM)
4	1	reservoir	ethylene glycol	20 (%(v/v))
5	1	reservoir	PEG3350	16-21 (%(w/v))