1GWG

Tri-iodide derivative of apoferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-15
Detector	MAR scanner 180 mm plate
Spacegroup name	F 4 3 2
Unit cell lengths	181.190, 181.190, 181.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.140 - 2.010
R-factor	0.169
Rwork	0.167
R-free	0.20500 *
Structure solution method	OTHER
RMSD bond length	0.019 *
RMSD bond angle	1.283 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.140	2.120
High resolution limit [Å]	2.010	2.010
Rmerge	0.073	0.321
Number of reflections	406056
<I/σ(I)>	10.1	2.4
Completeness [%]	98.9 *	93 *
Redundancy	9.1	6.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3		40MG/ML PROTEIN; 8MM CDSO4, 1M AMMONIUM SULPHATE, 0.01M NAN3., pH 5.30

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-40 (mg/ml)
2	1	drop		0.1 (M)
3	1	reservoir		7-12 (mM)
4	1	reservoir	ammonium sulfate	1 (M)
5	1	reservoir		3 (mM)