1GWB

STRUCTURE OF GLYCOPROTEIN 1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	P 64 2 2
Unit cell lengths	202.000, 202.000, 128.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.245
Rwork	0.245
R-free	0.27500 *
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.840
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.110 *	0.471 *
Number of reflections	37462
<I/σ(I)>	9.1
Completeness [%]	99.8 *	99.8 *
Redundancy	11.1 *	8.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	20 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH6.5
3	1	reservoir	ammonium sulfate	2 (M)