1GVN

Crystal Structure of the Plasmid Maintenance System epsilon/zeta: Meachnism of toxin inactivation and toxin function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	59.236, 79.847, 191.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.950
R-factor	0.2
Rwork	0.200
R-free	0.23500 *
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.110 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (V. 1.17)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.051	0.170
Number of reflections	64215
<I/σ(I)>	15.5	5.14
Completeness [%]	95.5	91.8
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	291 *	Meinhart, A., (2001) Acta Crystallogr., D57, 745. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.5
3	1	drop	dithiothreitol	5 (mM)
4	1	reservoir	sodium acetate	100 (mM)	pH4.6
5	1	reservoir	PEG3350	16 (%(w/v))