1GVJ
ETS-1 DNA BINDING AND AUTOINHIBITORY DOMAINS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-17 |
| Detector | RIGAKU IMAGE PLATE RAXIS V |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.475, 42.842, 49.771 |
| Unit cell angles | 115.25, 97.30, 102.40 |
Refinement procedure
| Resolution | 28.500 - 1.530 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1awc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.103 | 0.484 |
| Number of reflections | 40937 | |
| <I/σ(I)> | 15.648 | 2.021 |
| Completeness [%] | 93.9 | 87.1 |
| Redundancy | 3.103 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 297 | 0.2 M AMMONIUM ACETATE, 30% W/V PEG 4000, 0.1 M SODIUM CITRATE PH 5.6, PROTEIN CONCENTRATION 15 MG/ML PLUS 10 MM DTT, TEMPERATURE 297 K, FOR CRYOPROTECTION 10% OF PEG 400 WAS ADDED. |
