1GVD

CRYSTAL STRUCTURE OF C-MYB R2 V103L MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MAC SCIENCE M06XHF22
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-07-16
Detector	MAC Science DIP-2030
Spacegroup name	P 21 21 21
Unit cell lengths	28.951, 40.114, 48.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.450 - 1.450
R-factor	0.18
Rwork	0.180
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gv5
RMSD bond length	0.003
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.063	0.200
Number of reflections	10332
<I/σ(I)>	38.204	8.351
Completeness [%]	98.0	95.2
Redundancy	8.657	6.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	297	3.15 M AMMONIUM SULFATE IN 0.05 M MES BUFFER AT PH 6.8, PROTEIN CONCENTRATION 10 MG/ML PLUS 10 MM DTT, TEMPERATURE 297 K