1GVC

18kDa N-II domain fragment of duck ovotransferrin + NTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-01-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 31
Unit cell lengths	41.410, 41.410, 81.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.920 - 1.900
R-factor	0.186
Rwork	0.185
R-free	0.21100
Structure solution method	OTHER
RMSD bond length	0.013 *
RMSD bond angle	1.680 *
Data reduction software	MOSFLM
Data scaling software	Agrovata
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.920	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.041	0.088
Total number of observations	52160 *
Number of reflections	12081
<I/σ(I)>	8.5	7.6
Completeness [%]	98.0	97.3
Redundancy	4.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	SITTING DROP AT PH7.8 AND 277K, pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop	protein	20-40 (mg/ml)
3	1	reservoir	methanepentanediol	23 (%)