1GUX

RB POCKET BOUND TO E7 LXCXE MOTIF

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-07-07
Detector	ADSC QUANTUM
Spacegroup name	C 1 2 1
Unit cell lengths	103.500, 76.800, 64.000
Unit cell angles	90.00, 90.90, 90.00

Refinement procedure

Resolution	7.000 - 1.850
R-factor	0.218
Rwork	0.218
R-free	0.28900
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	21.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.940
High resolution limit [Å]	1.850	1.850
Rmerge	0.048 *	0.211 *
Total number of observations	135820 *
Number of reflections	45533
<I/σ(I)>	25.1	5.93
Completeness [%]	91.8	84.3
Redundancy	2.98	1.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	3.5 M SODIUM FORMATE, 0.1M NA HEPES PH 7.5, 20 MIROMOLAR CDCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	3.5 (M)
2	1	reservoir		0.02 (M)
3	1	reservoir	HEPES-Na	0.1 (M)