1GUP

STRUCTURE OF NUCLEOTIDYLTRANSFERASE COMPLEXED WITH UDP-GALACTOSE

Experimental procedure

Temperature [K]	123
Detector technology	MULTIWIRE AREA DETECTOR
Detector	BRUKER NONIUS HI-STAR
Spacegroup name	P 1 21 1
Unit cell lengths	68.700, 57.700, 188.700
Unit cell angles	90.00, 100.08, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.191 *
Rwork	0.191
RMSD bond length	0.013
RMSD bond angle	16.500 *
Data reduction software	SAINT
Data scaling software	XCALIBRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.047 *	0.099 *
Total number of observations	244754 *
Number of reflections	123193	13689 *
Completeness [%]	90.0	83 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG8000	11-13 (%)
3	1	drop	HEPES	50 (mM)
4	1	drop		100-150 (mM)
5	1	drop	UDP-sugar	5 (mM)
6	1	reservoir	PEG8000	22-26 (%)