1GUK
CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-09 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.300, 95.900, 50.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HGSTA1-1 DIMER (1GUH) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 24.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.076 | 0.319 |
| Number of reflections | 10477 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 80.0 | 75.8 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 20 * | CRYSTALLIZATION BY HANGING DROP VAPOR DIFFUSION RESERVOIR: 9% PEG 8000, 0.1 M TRIS/HCL PH 8.1, 5% MPD PROTEIN: 10 MG/ML DISSOLVED IN H20 DROP: 5 + 5 MICROLITER, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 4.5-6 (%) | |
| 3 | 1 | drop | MPD | 2.5-5 (%) | |
| 4 | 1 | drop | MES | 0.05 (M) | |
| 5 | 1 | reservoir | PEG8000 | 9-12 (%) | |
| 6 | 1 | reservoir | MPD | 5-10 (%) | |
| 7 | 1 | reservoir | MES | 0.1 (M) |
