1GU8

SENSORY RHODOPSIN II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	85.510, 128.553, 50.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 2.300 *
R-factor	0.23
Rwork	0.230
R-free	0.25500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h68
RMSD bond length	0.007
RMSD bond angle	1.140 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000 *	2.380
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.139	0.764
Total number of observations	76030 *
Number of reflections	13086
<I/σ(I)>	11.5	1.9
Completeness [%]	99.4	98.1
Redundancy	5.8	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		Royant, A., (2001) Proc. Natl. Acad. Sci. U.S.A., 98, 10131. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	4.5 (mg/ml)
2	1	1	n-dodecyl beta-D-maltoside	0.005 (%)
3	1	1		75 (mM)
4	1	1		1.5 (M)