1GTQ
6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 122.300, 122.300, 61.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.390 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.200 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.116 |
| Number of reflections | 22612 |
| Completeness [%] | 93.5 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 4 * | pH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | MES | 1 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
