1GTO

HIGH RESOLUTION STRUCTURE OF A HYPERSTABLE HELICAL BUNDLE PROTEIN MUTANT

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Detector technology	IMAGE PLATE
Collection date	1995-02-15
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	95.117, 95.117, 85.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.820
R-factor	0.224
Rwork	0.224
R-free	0.28000
RMSD bond length	0.006
RMSD bond angle	18.100 *
Data reduction software	DENZO (V. 1.3))
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.820 *	1.820 *
Rmerge	0.049	0.241 *
Number of reflections	17635 *
Completeness [%]	98.9 *	99.4 *
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8 *		Banner, D.W., (1987) J. Mol. Biol., 196, 657. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	2	phosphate	0.1 (M)
3	1	2		0.05 (M)
4	1	2	dithiothreitol	1 (mM)
5	1	2	MPD	30 (%)