1GTM

STRUCTURE OF GLUTAMATE DEHYDROGENASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Detector technology	IMAGE PLATE
Collection date	1994-09
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	167.200, 167.200, 172.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.17 *
Rwork	0.174
RMSD bond length	0.011
RMSD bond angle	2.850 *
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.043
Number of reflections	115181
Completeness [%]	93.2
Redundancy	2.58 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	290 *	Yip, K.S.P., (1995) Acta Crystallogr.,Sect.D, 51, 240. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	lithium sulfate	0.5-2.7 (M)
3	1	drop	HEPES	0.1 (M)