1GTL
The thermostable serine-carboxyl type proteinase, kumamolisin (KSCP) - complex with Ac-Ile-Pro-Phe-cho
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.030, 78.300, 73.170 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.800 |
| R-factor | 0.214 * |
| Rwork | 0.197 |
| R-free | 0.28400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ga1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.910 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | 0.205 |
| Total number of observations | 26276 * | |
| Number of reflections | 13434 | |
| <I/σ(I)> | 6.8 | 3.4 |
| Completeness [%] | 89.6 | 89.6 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | PH 4.50 |
