1GTK

Time-resolved and static-ensemble structural chemistry of hydroxymethylbilane synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	100
Detector technology	CCD
Collection date	1997-06-15
Spacegroup name	P 21 21 2
Unit cell lengths	87.500, 75.900, 50.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.850 - 1.660
R-factor	0.2
Rwork	0.198
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypn
RMSD bond length	0.021
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	O
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.850	1.750
High resolution limit [Å]	1.660	1.660
Number of reflections	34417
Completeness [%]	87.0	70.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3		PROTEIN WAS CRYSTALLISED AT PH 5.3 IN SITTING DROPS OF 0.05 ML WITH 6-7 MG/ML OF PROTEIN, 0.3 MM EDTA, 15 MM DITHIOTHREITOL, 10%(W/V) PEG6000 AND 0.01% NAN3 IN 0.1 M SODIUM ACETATE.