1GTE
DIHYDROPYRIMIDINE DEHYDROGENASE (DPD) FROM PIG, BINARY COMPLEX WITH 5-IODOURACIL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-26 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.710, 158.370, 162.330 |
Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
Resolution | 24.980 - 1.650 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DIHYDROPYRIMIDINE DEHYDROGENASE NON-LIGANDED FORM |
RMSD bond length | 0.009 |
RMSD bond angle | 1.380 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.047 | 0.238 |
Number of reflections | 481684 * | |
<I/σ(I)> | 15 | 4.3 |
Completeness [%] | 98.1 | 94.3 |
Redundancy | 3.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.7 | 293 * | Dobritzsch, D., (2001) Acta Crystallogr., 57, 153. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 17-22 (%(w/v)) | |
2 | 1 | reservoir | dithiothreitol | 10 (mM) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.7 |
4 | 1 | drop | DPD | 4.5 (mg/ml) |