1GTD

NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG ID TT50) STRUCTURE OF MTH169, THE PURS SUBUNIT OF FGAM SYNTHETASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Collection date	2001-09-15
Spacegroup name	P 43 2 2
Unit cell lengths	53.800, 53.800, 142.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.560
R-factor	0.235
Rwork	0.235
R-free	0.27800
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.157
Number of reflections	7888
<I/σ(I)>	14.9	3.2
Completeness [%]	94.0	54.2
Redundancy	5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		32%(V/V) MPD, 0.1M SODIUM CITRATE (PH 5.6) AND 0.2M AMMONIUM ACETATE