1GT7
L-rhamnulose-1-phosphate aldolase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 225.760, 225.760, 285.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.000 - 2.700 |
| R-factor | 0.233 * |
| Rwork | 0.233 |
| R-free | 0.23500 |
| Structure solution method | SIR |
| Data scaling software | SCALA |
| Phasing software | SCALEIT |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.089 | 0.370 * |
| Total number of observations | 611276 * | |
| Number of reflections | 207606 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 91.0 * | 90 * |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 * | HANGING DROP CONTAINED 5 MG/ML PROTEIN, 5 MM PGH, 0.9 M SODIUM FORMATE, 5 MM MERCAPTOETHANOL, 0.5 MM ZNCL2 AND 0.1 M SODIUM ACETATE (PH 4.6). RESERVOIR CONTAINED 1.8 M SODIUM FORMATE, 5 MM MERCAPTOETHANOL AND 0.1 M SODIUM ACETATE (PH 4.6) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | phosphoglycolohydroxamate | 5 (mM) | |
| 3 | 1 | drop | sodium formate | 0.9 (M) | |
| 4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | drop | 0.5 (mM) | ||
| 6 | 1 | drop | sodium acetate | 0.1 (M) | pH4.6 |
| 7 | 1 | reservoir | sodium formate | 1.8 (M) | |
| 8 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 9 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
