1GT5

Complexe of Bovine Odorant Binding Protein with benzophenone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.589, 65.206, 42.153
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	18.000 * - 2.080
R-factor	0.188
Rwork	0.188
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1obp
RMSD bond length	0.007
RMSD bond angle	25.500 *
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.150
High resolution limit [Å]	2.050 *	2.080
Rmerge	0.060 *	0.242 *
Number of reflections	17304
<I/σ(I)>	10.6	3.4
Completeness [%]	86.4 *	86.5
Redundancy	2.0 *	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4 *	4 *	pH 4.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ethanol	28-32 (%(v/v))
3	1	reservoir	citrate	50 (mM)	pH5.4