1GT4

Complex of Bovine Odorant Binding Protein with undecanal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.589, 65.206, 42.153
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	9.500 * - 2.100
R-factor	0.205
Rwork	0.205
R-free	0.25600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1obp
RMSD bond length	0.110 *
RMSD bond angle	1.640 *
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.060
High resolution limit [Å]	2.060 *	2.030
Rmerge	0.076 *	0.330 *
Number of reflections	17946
Completeness [%]	96.4	97.2
Redundancy	2.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4 *	4 *	pH 4.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ethanol	28-32 (%(v/v))
3	1	reservoir	citrate	50 (mM)	pH5.4