1GT3

Complex of Bovine Odorant Binding Protein with dihydromyrcenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.589, 65.206, 42.153
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.201
Rwork	0.201
R-free	0.23900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1obp
RMSD bond length	0.009
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.052	0.116
Number of reflections	28007
<I/σ(I)>	8.1	6.4
Completeness [%]	80.3	80.3
Redundancy	3.4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4 *	4 *	pH 4.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ethanol	28-32 (%(v/v))
3	1	reservoir	citrate	50 (mM)	pH5.4