1GT1

Complex of Bovine Odorant Binding Protein with Aminoanthracene and pyrazine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	55.589, 65.206, 42.153
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	10.000 - 1.710
R-factor	0.203
Rwork	0.203
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1obp
RMSD bond length	0.013
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.600 *	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.065 *	0.268 *
Number of reflections	30833
<I/σ(I)>	5.4	2.6
Completeness [%]	88.0	88
Redundancy	2.0 *	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4 *	4 *	pH 4.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ethanol	28-32 (%(v/v))
3	1	reservoir	citrate	50 (mM)	pH5.4