1GSK
Crystal structure of CotA, an endospore coat protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.051, 102.051, 136.393 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.710 - 1.700 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19850 * |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.837 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 0.252 |
| Number of reflections | 91214 | |
| <I/σ(I)> | 26.4 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 293 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 3 | 1 | reservoir | glycerol | 15 (%) | |
| 4 | 1 | reservoir | isopropanol | 12-15 (%) | |
| 5 | 1 | reservoir | PEG4000 | 12-15 (%) |
