1GSH

STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1991-03-18
Detector	RIGAKU
Spacegroup name	P 62 2 2
Unit cell lengths	87.800, 87.800, 170.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.208
Rwork	0.208
R-free	23.50000 *
RMSD bond length	0.012
RMSD bond angle	25.359 *
Data reduction software	WEIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.070
Number of reflections	30967
Completeness [%]	84.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		CRYSTALS WERE PREPARED AT PH 7.5 BY MICRODIALYSIS METHOD WITH AMMONIUM SULFATE AS THE PRECIPITATING AGENT. THE INNER SOLUTION 100 MICROLITER CONTAINED 1.5 % (W/V) GSHASE, 5 MM MGCL2 AND 10 % SATURATED AMMONIUM SULFATE IN 50 MM TRIS-HCL BUFFER (PH 7.5). THE INNER SOLUTION WAS DIALYZED AGAINST 50 MM TRIS-HCL BUFFER (PH 7.5) WHICH CONTAINED 5 MM MGCL2 AND 25 % SATURATED AMMONIUM SULFATE.TRIS-HCL BUFFER, microdialysis

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	50 (mM)
2	1	1		5 (mM)
3	1	1	ammonium sulfate	25 (%sat)