1GSA
STRUCTURE OF GLUTATHIONE SYNTHETASE COMPLEXED WITH ADP AND GLUTATHIONE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | FILM |
| Collection date | 1991-11-28 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 87.250, 87.250, 169.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.790 * |
| Data reduction software | WEIS |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.100 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.058 |
| Total number of observations | 196511 * |
| Number of reflections | 23410 |
| Completeness [%] | 81.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | 25 * | CRYSTALLIZED IN TRIS-HCL BUFFER (PH 7.5). AMMONIUM SULFATE WAS USED AS PRECIPITANT. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 2 | 1 | 1 | ammonium sulfate | 25 (%sat) | |
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | 2-mercaptoethanol | 10 (mM) | |
| 5 | 1 | 1 | 0.02 (%) |
