1GRW

C. elegans major sperm protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-05
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	53.478, 53.478, 457.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.228 *
Rwork	0.228
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2msp
RMSD bond length	0.013 *
RMSD bond angle	1.800 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (4.0.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000 *	2.560
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.072 *	0.359 *
Number of reflections	21887 *
<I/σ(I)>	6.3039	1
Completeness [%]	97.7 *	97.7 *
Redundancy	3.9 *	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	20 *	VAPOUR DIFFUSION AT 20DEGC IN 16% PEG 8000, 100 MM NA ACETATE BUFFER PH5.0, pH 5.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	16 (%(w/v))
2	1	reservoir	sodium acetate	100 (mM)	pH5.0