1GRP

REGULATORY AND CATALYTIC MECHANISMS IN ESCHERICHIA COLI ISOCITRATE DEHYDROGENASE: MULTIPLE ROLES FOR N115

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	105.100, 105.100, 150.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.199 *
Rwork	0.195
RMSD bond length	0.012
RMSD bond angle	2.800
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.118	0.298 *
Number of reflections	28004	1957 *
Completeness [%]	92.5	66.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	dephosphorylated IDH	28 (mg/ml)
2	1	1	ammonium sulfate	34 (%sat)
3	1	1		100 (mM)
4	1	1		100 (mM)
5	1	1	citric acid	9 (mM)
6	1	1	dithiothreitol	0.2 (mM)