1GRN

CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	120
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	52.400, 69.000, 129.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.211
Rwork	0.211
R-free	0.25900
Structure solution method	MAD
Starting model (for MR)	CDC42 IN THE GTP FORM
RMSD bond length	0.006
RMSD bond angle	22.570 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	1.800	2.100
Rmerge	0.064 *
Total number of observations	129060 *
Number of reflections	23543
<I/σ(I)>	29	9.3
Completeness [%]	85.9	85.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	15-20 (%(w/v))
2	1	reservoir	magnesium acetate	0.2 (M)
3	1	reservoir		0.10 (mM)
4	1	reservoir		10-30 (mM)