1GRL
THE CRYSTAL STRUCTURE OF THE BACTERIAL CHAPERONIN GROEL AT 2.8 ANGSTROMS
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 178.000, 203.000, 278.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.326 |
| Rwork | 0.326 |
| R-free | 0.36800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 117943 |
| Completeness [%] | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 28 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GroEL | 25 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 0.2 (%(w/v)) | |
| 3 | 1 | drop | ammonium sulfate | 0.86 (M) | |
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | drop | Tris-acetate | 50 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 0.4 (%(w/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.72 (M) | |
| 8 | 1 | reservoir | 4 (mM) | ||
| 9 | 1 | reservoir | Tris-acetate | 100 (mM) |
