1GRJ

GREA TRANSCRIPT CLEAVAGE FACTOR FROM ESCHERICHIA COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	IMAGE PLATE
Collection date	1994-01-04
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	102.357, 42.660, 39.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.209
Rwork	0.209
R-free	0.32000
RMSD bond length	0.018
RMSD bond angle	2.100
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.200
Rmerge	0.072 *
Total number of observations	30813 *
Number of reflections	8079
Completeness [%]	85.5 *
Redundancy	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	18-20 (%(w/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	dithiothreitol	10 (mM)
4	1	reservoir	MES	0.1 (M)