1GQP

APC10/DOC1 SUBUNIT OF S. cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	91.065, 91.065, 114.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.200
R-factor	0.2109 *
Rwork	0.213
R-free	0.24500 *
Structure solution method	MIRAS
RMSD bond length	0.011
RMSD bond angle	1.375 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.058	0.278 *
Total number of observations	310219 *
Number of reflections	27256
<I/σ(I)>	22.9
Completeness [%]	99.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	20 *	0.1M SODIUM ACETATE, 0.3M LITHIUM BROMIDE AND 2M SODIUM FORMATE, PH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.1 (M)
2	1	reservoir	sodium formate	2 (M)	pH4.6
3	1	reservoir	glycerol	15 (%(v/v))
4	1	reservoir		0.3 (M)