1GQL
Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid and xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.256, 74.323, 87.273 |
| Unit cell angles | 115.15, 92.94, 109.24 |
Refinement procedure
| Resolution | 20.000 - 1.670 |
| R-factor | 0.146 |
| Rwork | 0.146 |
| R-free | 0.17700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE ALPHA-D-GLUCURONIDASE |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.724 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.049 | 0.225 |
| Number of reflections | 149092 * | 6204 * |
| <I/σ(I)> | 15.7 | 3 |
| Completeness [%] | 93.9 | 86.4 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | 30MG/ML, 15% PEG3350, 250MM MGCL2, 5MM TRIS PH8.0, 20% ETHYLENE GLYCOL, 50MM GLUCURONIC ACID, 50MM XYLOTRIOSE, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
| 5 | 1 | reservoir | PEG3350 | 15 (%(w/v)) |
