1GQK
Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.707, 74.879, 87.481 |
| Unit cell angles | 115.34, 93.06, 109.20 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE ALPHA-D-GLUCURONIDASE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.353 |
| Number of reflections | 113754 | 5509 * |
| <I/σ(I)> | 9.8 | 2.2 |
| Completeness [%] | 97.3 | 94.1 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | 30MG/ML, 15% PEG3350, 250MM MGCL2, 5MM TRIS PH8.0, 20% ETHYLENE GLYCOL 100MM GLUCURONIC ACID, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
| 5 | 1 | reservoir | PEG3350 | 15 (%(w/v)) |
