1GQE
Polypeptide Chain Release Factor 2 (RF2) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.388, 49.893, 63.144 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.800* |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.24700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.319 * |
| Total number of observations | 1018104 * | |
| Number of reflections | 58787 * | |
| <I/σ(I)> | 12.3 | 1.7 |
| Completeness [%] | 95.9 * | 71.2 * |
| Redundancy | 2.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 | 20 * | 40 MM TRISHCL, PH 7.6, 400 MM NACL, 40 MM MGCL2, 2% ETHYLENE GLYCOL 20 MM DTT, PEG 2K MME 28-34% |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 40 (mM) | pH7.6 |
| 2 | 1 | reservoir | 400 (mM) | ||
| 3 | 1 | reservoir | 40 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 2 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 6 | 1 | reservoir | PEG2000 MME | 28-34 (%) | |
| 7 | 1 | drop | protein | 3.5 (mg/ml) | and 8.0mg/ml |
