1GPI
Cellobiohydrolase Cel7D (CBH 58) from Phanerochaete chrysosporium. Catalytic module at 1.32 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.307, 46.625, 99.079 |
| Unit cell angles | 90.00, 103.19, 90.00 |
Refinement procedure
| Resolution | 13.000 - 1.320 |
| R-factor | 0.1875 * |
| Rwork | 0.213 |
| R-free | 0.22690 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cel |
| RMSD bond length | 0.024 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 1.340 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.028 | 0.206 |
| Number of reflections | 89559 | |
| <I/σ(I)> | 17.2 | 4 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 22.5% PEG5000, 5 MM CACL2, 10 MM TRIS-HCL, PH 7.0, 12.5% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | R-propanolol | 12.5 (mM) | |
| 3 | 1 | drop | sodium acetate | 1 (mM) | pH5.0 |
| 4 | 1 | reservoir | PEG5000 MME | 27.5 (%(w/v)) | |
| 5 | 1 | reservoir | 2 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 10 (mM) | pH7.0 |
| 7 | 1 | reservoir | glycerol | 10 (%(v/v)) |
