1GPC
CORE GP32, DNA-BINDING PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Collection date | 1994-01-15 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.500, 66.500, 235.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.016 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.058 |
| Total number of observations | 33415 * |
| Number of reflections | 16406 |
| Completeness [%] | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.4 * | MOLECULE: CORE GP32. CRYSTALLIZED IN PRESENCE OF P(DT6). |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 20 (%) | |
| 2 | 1 | 1 | ammonium sulfate | 100 (mM) | |
| 3 | 1 | 1 | sodium chloride | 50 (mM) | |
| 4 | 1 | 1 | ethylene glycol | 5 (%) | |
| 5 | 1 | 1 | EPPS | 20 (mM) |
