1GOS
Human Monoamine Oxidase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 138.800, 224.300, 87.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.27100 |
| Structure solution method | SIR |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.128 | 0.338 * |
| Number of reflections | 24034 | |
| Completeness [%] | 95.3 | 94.6 * |
| Redundancy | 8.1 | 6.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 25 (mM) | pH7.5 |
| 3 | 1 | drop | Zwittergent | 8.5 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
| 5 | 1 | reservoir | ADA | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | lithium sulfate | 70 (mM) |
