Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.220, 59.240, 51.310 |
| Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.140 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 1.150 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.078 | 0.238 |
| Number of reflections | 80691 | |
| <I/σ(I)> | 9.82 | 3.15 |
| Completeness [%] | 85.2 | 78.8 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROPS CONTAINING 1:1 RATIO OF 20 MG/ML PROTEIN SOLUTION AND RESERVOIR SOLUTION (12 % TO 25 % PEG 6,000)., pH 7.00 |
