1GOJ
Structure of a fast kinesin: Implications for ATPase mechanism and interactions with microtubules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.970, 72.730, 84.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.219 * |
| Rwork | 0.223 |
| R-free | 0.25600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kin |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 23.300 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.319 |
| Total number of observations | 138093 * | |
| Number of reflections | 14829 | |
| <I/σ(I)> | 15.8 | 6.3 |
| Completeness [%] | 99.8 | 100 * |
| Redundancy | 8. * | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 19 * | 100 MM HEPES PH 6.5-7.5, 17.5% PEGMME2000, 3% GLYCEROL, PROTEIN CONCENTRATION = 7.5 - 15 MG/ML |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 17.5 (%) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH6.5-7.5 |
| 3 | 1 | reservoir | glycerol | 3 (%) | |
| 4 | 1 | drop | protein | 7.5-15 (mg/ml) |
