1GOD

MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-01-15
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	60.560, 60.560, 84.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.188 *
Rwork	0.188
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1clp
RMSD bond length	0.015
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.800
Rmerge	0.090 *
Total number of observations	22540 *
Number of reflections	4482 *
Completeness [%]	99.5
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6		0.1 M SODIUM ACETATE (PH 4.6), 2.0 M (NH4)2SO4, 1MM NANO3, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	acetate	0.1 (M)	pH4.6
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	reservoir		1 (mM)