1GNW
STRUCTURE OF GLUTATHIONE S-TRANSFERASE
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 63 |
| Unit cell lengths | 113.300, 113.300, 69.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.028 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | |
| High resolution limit [Å] | 2.200 * | 2.200 * |
| Rmerge | 0.094 | |
| Number of reflections | 23438 | |
| Completeness [%] | 88.9 | 46.2 * |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | 0.02 (%) | ||
| 2 | 1 | drop | Hepes-NaOH | 6.7 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | drop | ammonium sulfate | 0.93 (M) | |
| 5 | 1 | drop | PEG4000 | 0.067 (%) | |
| 6 | 1 | drop | S-heylglutathione | 5.0 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 8 | 1 | reservoir | PEG4000 | 0.2 (%(w/v)) | |
| 9 | 1 | reservoir | Hepes-NaOH | 0.2 (M) |
