1GNT
Hybrid Cluster Protein from Desulfovibrio vulgaris. X-ray structure at 1.25A resolution using synchrotron radiation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.810, 64.530, 151.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.250 |
| R-factor | 0.157 * |
| Rwork | 0.157 |
| R-free | 0.17400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB FROM ENTRY 1E2U |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.462 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0.36) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 1.340 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.063 * | 0.331 * |
| Total number of observations | 400513 * | |
| Number of reflections | 163543 | |
| <I/σ(I)> | 6.2 | 1.6 |
| Completeness [%] | 94.6 | 86.4 |
| Redundancy | 2.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 277 * | 25-30% PEG 8000 0.1M MES PH 5.9, 0.2M MAGNESIUM ACETATE, T=277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 0.1 (M) | pH5.9 |
| 2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | PEG8000 | 25-30 (%) | |
| 4 | 1 | drop | protein | 12 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
